Analytics
Stable adducts of nerve agents with buffer compounds like TRIS and HEPES
A new publication in the Journal of Chromatography B describes the formation of stable adducts of nerve agents like Sarin, Soman or Cyclosarin and common buffer compounds like TRIS,TES or HEPES. The molecular structure of the adducts was determined by LC-ESI-MS/MS and NMR spectroscopy. They were found to be phosphodiesters (“O-adducts”). Alternative buffering compounds for the work with warfare agents are proposed.
Reaction monitoring with 1H-31P HSQC NMR spectroscopy
A new publication in Analytical and Bioanalytical chemistry describes the use of 1H-31P HSQC NMR spectroscopy to monitor the degradation of highly toxic organophosphorus compounds by the enzyme DFPase.The method can be used for methylphosphonates, a group of compounds including nerve agents sarin (GB), soman (GD), cyclosarin (GF) and also VX. The limit of quantitation (LOQ) of the method is around 100 μM when using a 400 MHz NMR spectrometer.
Reaction Monitoring with FTIR Spectroscopy
A new publication describes the use of in-situ FTIR spectroscopy to monitor the degradation of highly toxic organophosphorus compounds by the enzyme DFPase. The use of Attenuated Total Reflexion (ATR) allows direct meassurements in the reaction vessel without the need for cuvettes. In comparison to established methods the total reaction volume can be significantly reduced, which also leads to a substantial reduction in the required ammount of toxic substrate and therefore to an increase in work safety.
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