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Reaction Monitoring with FTIR Spectroscopy

A new publication describes the use of in-situ FTIR spectroscopy to monitor the degradation of highly toxic organophosphorus compounds by the enzyme DFPase. The use of Attenuated Total Reflexion (ATR) allows direct meassurements in the reaction vessel without the need for cuvettes. In comparison to established methods the total reaction volume can be significantly reduced, which also leads to a substantial reduction in the required ammount of toxic substrate and therefore to an increase in work safety.