Stable adducts of nerve agents with buffer compounds like TRIS and HEPES

A new publication in the Journal of Chromatography B describes the formation of stable adducts of nerve agents like Sarin, Soman or Cyclosarin and common buffer compounds like TRIS,TES or HEPES. The molecular structure of the adducts was determined by LC-ESI-MS/MS and NMR spectroscopy. They were found to be phosphodiesters (“O-adducts”). Alternative buffering compounds for the work with warfare agents are proposed.